<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="6.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Eaton, Heather</style></author><author><style face="normal" font="default" size="100%">Penny, Emily</style></author><author><style face="normal" font="default" size="100%">Brunetti, Craig</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Antibody dependent enhancement of frog virus 3 infection</style></title><secondary-title><style face="normal" font="default" size="100%">Virology Journal</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">antibody</style></keyword><keyword><style  face="normal" font="default" size="100%">frogs</style></keyword><keyword><style  face="normal" font="default" size="100%">Iridoviridae</style></keyword><keyword><style  face="normal" font="default" size="100%">Ranavirus</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2010</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.virologyj.com/content/7/1/41</style></url></web-urls><related-urls><url><style face="normal" font="default" size="100%">http://www.amphibiatree.org/sites/amphibiatree.org/files/EatonETAL2010_Ranavirus.pdf</style></url></related-urls></urls><number><style face="normal" font="default" size="100%">1</style></number><volume><style face="normal" font="default" size="100%">7</style></volume><pages><style face="normal" font="default" size="100%">41</style></pages><isbn><style face="normal" font="default" size="100%">1743-422X</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">BACKGROUND:Viruses included in the family Iridoviridae are large, icosahedral, dsDNA viruses that are subdivided into 5 genera. Frog virus 3 (FV3) is the type species of the genus Ranavirus and the best studied iridovirus at the molecular level. Typically, antibodies directed against a virus act to neutralize the virus and limit infection. Antibody dependent enhancement occurs when viral antibodies enhance infectivity of the virus rather than neutralize it.RESULTS:Here we show that anti-FV3 serum present at the time of FV3 infection enhances infectivity of the virus in two non-immune teleost cell lines. We found that antibody dependent enhancement of FV3 was dependent on the Fc portion of anti-FV3 antibodies but not related to complement. Furthermore, the presence of anti-FV3 serum during an FV3 infection in a non-immune mammalian cell line resulted in neutralization of the virus. Our results suggest that a cell surface receptor specific to teleost cell lines is responsible for the enhancement.CONCLUSIONS:This report represents the first evidence of antibody dependent enhancement in iridoviruses. The data suggests that anti-FV3 serum can either neutralize or enhance viral infection and that enhancement is related to a novel antibody dependent enhancement pathway found in teleosts that is Fc dependent.</style></abstract><accession-num><style face="normal" font="default" size="100%">doi:10.1186/1743-422X-7-41</style></accession-num></record></records></xml>
